Sero-surveillance of Newcastle Disease Virus in Kuroilers and Indigenous Birds in Darjeeling District
نویسندگان
چکیده
Kuroilers are semi-synthetic, dual purpose breed of birds with higher productivity than indigenous breeds and are commonly reared in hilly area like Darjeeling district of West Bengal. The present study was intended to detect the sero-prevalence of Newcastle disease virus in kuroilers and indigenous birds in Darjeeling district of West Bengal. The serum samples from kuroilers (n=10) and indigenous birds (Deshi or non-descript chicken, n=17) were collected from different regions of Darjeeling district following the standard procedure. Unvaccinated birds were selected for the study. An indirect ELISA was performed by using a kit for detection of NDV antibodies following the manufacturer’s instructions. In total, 22 samples (22/27, 81.4%) (8 from Kuroiler and 14 from indigenous birds) showed the antibody titer above the cut off value which was considered as positive as per the manufacturer’s instruction.
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